Neb provides high quality recombinant taq at an exceptional value. Template purity and quality are also critical to pcr success. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr. The onetaq reaction buffers and high gc enhancer have been formulated for robust yields with minimal. However, all versions of taq polymerase are deficient in two respects. Taq with standard taq buffer is available in economical extralarge pack sizes. Taq dna polymerase taq pol i isolated from thermus aquaticus has been shown to be highly useful in the polymer ase chain reaction pcr method 1, 2 of amplifying dna fragments 3.
Platinum taq dna polymerase is recombinant taq dna polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Usb taq is functionally tested and has no detectable contaminating. Platinum ii taq hotstart dna polymerase is designed for universal primer annealing and fast, easy pcr with its unique combination of innovative buffer, highperformance engineered taq dna polymerase, and leading hotstart technology. Hot start taq dna polymerase multiplex pcr 5x master mix onetaq 2x master mix with standard buffer onetaq dna polymerase onetaq hot start 2x master mix with gc buffer onetaq hot start 2x master mix with standard buffer onetaq hot start dna polymerase onetaq hot start quickload 2x master mix with gc buffer onetaq hot start quick. Platinum taq dna polymerase from thermo fisher scientific. Mb000042eut0 is a thermostable dna polymerase purified from an e. If desired, a master mix can be prepared for multiple reactions, to. Alliance bio taq dna polymerase is a thermostable recombinant dna polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq dna polymerase is the most common polymerase used for pcr reactions applications taq dna polymerase can be used in most applications including the following. Gotaq dna polymerase m300 protocolpdf 207 kb english.
Taq dna polymerase is a thermostable enzyme derived from thermus aquaticus which is purified from e. In some cases, additives can enhance pcr efficiency, specificity, and yield. Scientists realized that thermostable heatstable dna polymerases would be needed for pcr to work efficiently. Taq dna polymerase is appropriate for use in the amplification of dna from complex genomic, viral, and plasmid templates, rtpcr, sequencing ssdna, and cycle sequencing unit definition. Taq polymerase, being thermostable, proved ideal for pcr.
This enzyme possesses a highly processive 53 polymerase activity and a 53 exonuclease activity1. E331, e332, se332, me332, le332 depending on the quantity. Taq dna polymerase is a thermostable enzyme that synthesizes dna from singlestranded templates in the presence of dntps and a primer. It is supplied with 10x thermopol reaction buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.
Onetaq dna polymerase is an optimized blend of taq and deep vent dna polymerases for use with routine and difficult pcr experiments. Taq polymerase has substantial enzymatic activity at 37c, although its optimal activity is expressed at a much higher temperature approximately 72c. Can taq dna polymerase use rna as a template, and generate. In some cases, more enzyme may be required up to 2. The recommended starting amount is 5% blood added directly to the reaction without further modification.
Invitrogen platinum taq dna polymerase is a convenient and reliable hot start thermostable dna polymerase for pcr that provides enhanced specificity over that of taq dna polymerase. Taq dna polymerases product listing product overview. Ampliqon taq dna polymerase has a molecular weight of 95 kda and exhibits both a 53 dna polymerase and a 53 exonuclease activity. Prior to use, dilute the low glycerol platinum taq, cg dna polymerase 50 u. High fidelity is provided by a mixture of platinum taq dna polymerase and the proofreading 3. If amplification does not give satisfactory results. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr denaturation step. Platinum hotstart technology inhibits dna polymerase activity at ambient temperatures, allowing room temperature reaction setup and storage of preassembled pcr reactions for up to 24 hours prior to the pcr. This enzyme has a 5 3 dna polymerase and a 5 3 exonuclease activity but lacks a. Taq dna polymerase is a thermostable enzyme derived from the thermophilic bacterium thermus aquaticus. Enzyme activity is restored after the initial denaturation step.
Platinum superfi dna polymerase has 5 to 3 polymerase and. Platinum taq dna polymerase high fidelity contains recombinant taq dna polymerase, pyrococcus species gbd polymerase, and platinum taq antibody. Invitrogen platinum ii taq hotstart dna polymerase is an engineered taq dna polymerase that shows increased resistance to reaction inhibitors originating from sample material or dna purification steps. It is frequently used in the polymerase chain reaction pcr, a method for greatly amplifying the quantity of short segments of dna. Aug 17, 1999 the dna polymerase i from thermus aquaticus taq polymerase has been used extensively in pcrs to amplify small quantities of dna. L to at least 10 ul in low glycerol platinum taq, cg diluent included in the kit.
Taq dna polymerase buffered aqueous glycerol solution. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kda. Taq dna polymerase is an enzyme widely used in pcr 7. Taq dna polymerase is designed for the sensitive, reproducible, endpoint detection and analysis of rna molecules by rtpcr. Use platinum taq dna polymerase for the amplification of dna from complex genomic, viral, and plasmid templates, as well as in rtpcr. Taq plus dna polymerase is a mixture of taq dna polymerase and pfu, a proofreading dna polymerase, which allows for the amplification of long templates, up to 20kb, with high fidelity. For example, taq with standard taq buffer is designed to support existing pcr platforms. The polymerase activity is blocked at ambient temperatures and restored after the initial denaturation step at 94c. The 53 exonuclease activity leaves a 3da overhang on the pcr product, which are convenient for direct ta cloning. In addition, the availability of the enzyme and the dna sequence of the taq dna polymerase gene will facilitate the. High fidelity dna synthesis by the thermus aquaticus dna polymerase. Description the superscriptz iii onestep rtpcr system with platinum. Because of its high turnover number, lack of a proofreading activity, hightemperature optimum, and ability to incorporate 7deaza3deoxyguanosine efficiently, taq dna polymerase also has been used extensively for dna sequencing. Optimization of the polymerase chain reaction with regard to fidelity.
Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus, from which it was originally isolated by chien et al. Taq excels at amplifying shorter taq dna polymerase. The taq enzyme has 53 polymerase activity, doublestrand specific 53 exonuclease activity, and 3 adenylation activity. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr denaturation step, thus preventing the extention of.
This enzyme allows amplification of simple and complex dna templates over a large range of target sizes and provides 6x higher fidelity over taq. Taq dna polymerase is the industry standard for routine pcr. Superscriptz iii onestep rtpcr system with platinum. The enzyme consists of a single polypeptide with a molecular weight of 94 kda. Cycling times for each polymerase are shown in purple, while ramping times on the proflex pcr system 6. Direct pcr from blood using platinum ii taq hotstart dna. The dna fragment was amplified from a 5fold serial dilution of l dna with an initial concentration of 0. Platinum ii taq hotstart dna polymerase can amplify dna from a wide range of blood concentrations. After dilution, the low glycerol platinum taq, cg dna polymerase 10 ul can be stored at 4c for use within 12 days. Recombinant taq dna polymerase is the enzyme of choice for most pcr applications. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr denaturation step convenience. Therefore, we sought to clone the taq pol i gene and express the gene in e. Taq dna polymerase is a thermostable dna polymerase that catalyzes the polymerization of nucleotides into duplex dna in the 5 3 direction. Taq is available with different formats to accommodate a variety of pcr applications.
Difference between taq polymerase and dna polymerase. The following guidelines are provided to ensure successful. Taq dna polymerase has an intrinsic rnadependent dna polymerase activity reverse transcriptase activity. Pcr kit, b neb onetaq hot start dna polymerase, c promega gotaq g2 dna polymerase, d toyobo quick taq hs dyemix, e roche faststart taq dna polymerase, and f sigmaaldrich jumpstart taq dna polymerase. Mar 02, 2017 taq dna polymerase is one of a dna polymerase enzyme which is highly useful in polymerase chain reaction pcr method of dna amplification. The polymerase chain reaction pcr was developed by chemist kary mullis in the 1980s, as a means to make many copies of dna fragments. Platinum taq dna polymerase thermo fisher scientific. Buffer, dna template, primers, 200 m dntps not included and 1. Pcr fidelity of pfu dna polymerase and other thermostable. Platinum taq dna polymerase, high fidelity, is ideal for amplification of dna fragments when high yields and robust amplification are required. Sep 15, 1996 ling ll, keohavong p, dias c, thilly wg.
It is isolated from a heatloving bacterium that is naturally found in hot springs, so the enzyme doesnt break down at the high temperatures necessary. Datasheet for taq dna polymerase with standard taq buffer neb. Taq dna polymerase is the original and most commonly used pcr enzyme. Taq dna polymerase and taq pcr core kit en print bookmark share pdf 63kb english format. Sequence and primer concentrations also determine overall.
Taq dna polymerase is a thermostable dna polymerase that possesses a 5. The two enzymes act synergistically during pcr to generate more accurate. It is supplied with 10x standard taq reaction buffer, which is detergentfree and designed to be compatible with existing assay systems. Taq dna polymerase is a thermostable dna polymerase isolated from an e. Mytaq dna polymerase is a high performance polymerase that exhibits more robust amplification than other commonly used polymerases, delivering very high yield over a wide range of pcr templates and making it the ideal choice for most pcr assays.
Bioneers taq dna polymerase is isolated from recombinant li strain containing the dna polymerase gene from thermus aquaticus yt1. Structurebased design of taq dna polymerases with improved. Mangotaq dna polymerase is a formulation of taq dna polymerase that offers highyield across a wide range of dna concentrations mangotaq dna polymerase possesses 5. Pcr fidelity of pfu dna polymerase and other thermostable dna. Enhanced performance over standard taq dna polymerase. Activity is restored after the denaturation step in pcr cycling at 94c, thereby providing an automatic hot start for taq dna polymerase in pcr 1,2,3. This enzyme has a 5 3 dna polymerase and a 5 3 exonuclease activity but lacks a 3 5 exonuclease activity. Pcr optimization the choice of the pcr enzyme in combination with an appropriate buffer can profoundly affect pcr outcome. The high temperature optimum activity, 75 c, affords unique advantages when comparing taq pol i to escherichia coli dna polymerase i. Features of platinum ii hotstart pcr master mix 2x include. Platinum taq dna polymerase is a convenient and reliable hot start thermostable dna polymerase for pcr that provides enhanced specificity over that of taq dna polymerase. Avoid extensive mixing or vortexing after addition of blood to the pcr mix. Taq dna polymerase is a highly thermostable dna polymerase of the thermophilic bacterium thermus aquaticus.
The taq dna polymerase is the most successful application of a product derived from an extremophile, with annual sales around half a billion dollars podar and reysenbach, 2006. One unit of taq dna polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into dna in 30 minutes at 74c. It has a 53 dna polymerase activity and a 53 exonuclease activity. The taq dna polymerase is the most commonly used enzyme in dna sequencing. Taq dna polymerase and taq pcr core kit en print bookmark share pdf 63kb english format file size language download get adobe reader.
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